BMB514: Immunological techniques

Study Board of Science

Teaching language: Danish
EKA: N200007102
Assessment: Second examiner: None
Grading: Pass/Fail
Offered in: Odense
Offered in: Autumn
Level: Bachelor

STADS ID (UVA): N200007101
ECTS value: 5

Date of Approval: 25-04-2019


Duration: 1 semester

Version: Approved - active

Comment

A course Manual of 130 pages consisting of a schematic presentation of the overall themes, a table of contents, a detailed course programme with colour coding refers to the schematic presentations and the actual manual consisting of 13 chapters with a theoretical introduction and a detailed description of the individual exercises.

Limitation of participants:
The course has limited seats and in case of too many applications, priority is given to the timely registration by date of registration. 
The allocation of the course 120 places is up to 80 places for biomedicine and biochemistry and molecular biology students. However, biomedicine students have first priority for the course, as the course is compulsory for them. If the number of enrolled biomedicine students exceeds 80, the number of places for pharmacy students will be reduced accordingly.

Entry requirements

None

Academic preconditions

Knowledge of BMB512 or SU518 or equivalent courses.

Participant limit

120

Course introduction

To give the student practical experience in immunological techniques and the production and characterization of immunoreactants, and to provide them with a grasp of the great potential of these methods in biological research and development.

Expected learning outcome

When the course is completed, the student is expected to:
  • have gained a better understanding of theoretical immunology, through "hands-on” experience of immune system
  • be able to manufacture, purify and characterise antibodies produced in, for example, rabbit or mouse
  • use antibodies for qualitative and quantitative analysis
  • be able to develop optimise and validate immunological assays
  • be able to determine qualitative parameters such as antigen specificity, epitope specificity, affinity and the involvement of the types of bonds in the reaction between antigen and antibody
  • work with cell cultures in the manufacture and characterisation of monoclonal antibodies
  • work in accordance to regulations in a sterile laboratory
  • assess the results of tests based on the use of immunological reagents.

Content

I. Production of monoclonal antibodies, including immunisation, fusion, selection, cloning and the determination of specificity.

II. Analysis of polyclonal antibodies, purification of IgG, analysis of purity and specificity and the production of mono-specific preparations from poly-specific antisera.

III. Quantitative immunological techniques with special focus on the "sandwich" format in the form of Enzyme-Linked Immunosorbent Assay (ELISA). With regard to ELISA, skills in developing and configuring the assay will be acquired and applied to the quantification of an antigen in various body fluids. The effects of interference by heterophilic anti-Ig antibodies and human anti-animal IgG antibodies will also be examined. In addition, quantitative radio-immunoassay (RIA) and quantitative immunoelectrophoresis and immunodiffusion techniques will be performed.

IV. Analytical immunological techniques. In connection with the production of monoclonal antibodies, students will be instructed about the setup and application of analytical ELISA to identify relevant hybridomas and to monitor cloning. The students are introduced to the use of analytical immunoelectrophoresis techniques to study the specificity of polyclonal antibodies and to monitor chromatographic separations. The students will also be schooled in the use of double immunodiffusion to illustrate the concepts of immunological identity, non-identity and cross-reaction. Western blots will be performed in relation to the identification of specific antigens in a biological fluid and to evaluation of the specificity of the antibodies employed. In the course of the ELISA exercises, knowledge about new technologies to define the relative epitope specificity of monoclonal antibodies will also be acquired.

V. In the course teaches students the use of fluorochrome-labelled antibodies in FACS analysis to identify specific cell types and quantification of receptors on these cells. In addition, students are trained in the use of immunohistochemistry to test antigen localization in tissue sections, using enzyme-labelled antibodies.

VI. Students will be instructed in the immunoprecipitation, isolation and characterization of radioactive-labelled protein mixtures, using specific antibodies in combination with SDS-PAGE and autoradiography. Introduction to immunoagglutination will be acquired through exercises centred on the AB0 blood group system.

Literature

Exercise instructions for the course
See itslearning for syllabus lists and additional literature references.

Examination regulations

Exam element a)

Timing

Autumn

Tests

Rapport

EKA

N200007102

Assessment

Second examiner: None

Grading

Pass/Fail

Identification

Full name and SDU username

Language

Normally, the same as teaching language

Examination aids

To be announced during the course

ECTS value

5

Indicative number of lessons

60 hours per semester

Teaching Method

At the faculty of science, teaching is organized after the three-phase model ie. intro, training and study phase.
Laboratory exercises, 10 days with 6 hours per day.

Teacher responsible

Name E-mail Department
Yaseelan Palarasah ypalarasah@health.sdu.dk Cancer & Inflammationsforskning - Inflammation

Additional teachers

Name E-mail Department City
Anders Grønnegaard Schlosser aschlosser@health.sdu.dk Cancer & Inflammationsforskning - Inflammation
Jonas Heilskov Graversen jgraversen@health.sdu.dk Cancer & Inflammationsforskning - Inflammation
Karsten Skjødt kskjoedt@health.sdu.dk Cancer & Inflammationsforskning - Inflammation
Maiken Lumby Henriksen mlhenriksen@health.sdu.dk Cancer & Inflammationsforskning - Inflammation
Søren Hansen SHansen@health.sdu.dk Cancer & Inflammationsforskning - Inflammation

Timetable

Administrative Unit

Biokemi og Molekylær Biologi

Team at Educational Law & Registration

NAT

Offered in

Odense

Recommended course of study

Profile Education Semester Offer period
BSc in major Biomedicine - registration 1 september 2016 og 2017 | Odense 5 E20
BSc in major Biomedicine - registration 1 september 2016 og 2017 | Odense 5 E19
BSc in major Biomedicine - registration 1 september 2018 | Odense 5 E19
BSc in major Biomedicine - registration 1 september 2018 | Odense 5 E19
BSc in major Biomedicine - registration 1 september 2018 | Odense 5 E21
BSc in major Biomedicine - registration 1 september 2018 | Odense 5 E20
BSc in major Biomedicine - registration 1 september 2018 | Odense 5 E20
BSc in major Biomedicine - registration 1 september 2018 og 2019 | Odense 5 E21
BSc in major Biomedicine - registration 1 september 2019 | Odense 5 E22
BSc in major Biomedicine - registration 1 september 2020 | Odense 5 E20
BSc in major Biomedicine - registration 1 september 2020 and 2021 | Odense 5 E21
BSc in major Biomedicine - registration 1 september 2020 and 2021 | Odense 5 E22
BSc in major Biomedicine - registration 1 september 2020 and 2021 | Odense 5 E23
BSc in major Biomedicine - registration 1 september 2022 | Odense 5 E22
BSc in major Biomedicine - registration 1 september 2022 and 2023 | Odense 5 E23
BSc major in Pharmacy - Registration 1 September 2018 and 2019 Bachelor of Science (BSc) in Pharmacy | Odense 5 E21
BSc major in Pharmacy - Registration 1 September 2019 Bachelor of Science (BSc) in Pharmacy | Odense 5 E22
BSc major in Pharmacy - Registration 1 September 2020 and 2021 Bachelor of Science (BSc) in Pharmacy | Odense 5 E21
BSc major in Pharmacy - Registration 1 September 2020, 2021 and 2022 Bachelor of Science (BSc) in Pharmacy | Odense 5 E22
BSc major in Pharmacy - Registration 1 September 2020, 2021, 2022 and 2023 Bachelor of Science (BSc) in Pharmacy | Odense 5 E23

Transition rules

Transitional arrangements describe how a course replaces another course when changes are made to the course of study. 
If a transitional arrangement has been made for a course, it will be stated in the list. 
See transitional arrangements for all courses at the Faculty of Science.