BMB530: Basic Biochemistry
Study Board of Science
Teaching language: Danish or English depending on the teacher
EKA: N200009112, N200009102, N200009122
Assessment: Second examiner: None, Second examiner: External
Grading: Pass/Fail, 7-point grading scale
Offered in: Odense
Offered in: Autumn
Level: Bachelor
STADS ID (UVA): N200009101
ECTS value: 5
Date of Approval: 27-03-2019
Duration: 1 semester
Version: Archive
Comment
Academic preconditions
Students taking the course are expected to:
- Have knowledge of biology, chemistry and data analysis
- Be able to use Word and Excel
- Be able to prepare an experimental exercise based on guidance from a protocol
Course introduction
To give the students a basic introduction to the catalytic function and mechanisms of enzyme action, to the structure and function of biological membranes and a basic introduction to eukaryotic cell metabolism on a molecular level. The students are introduced to the underlying theory of enzymes and membranes and in the methods used to study these. In addition some biochemical processes in animal and plant metabolism and their regulation are introduced with an emphasis on mammalian cells.
The course builds on the knowledge acquired in the courses FF503, and gives an academic basis for studying the topics of cellular biology in relation to degrees within pharmacology, chemistry and biology.
In relation to the competence profile of the degree it is the explicit focus of the course to:
- Use methods of enzyme kinetics and biochemistry in theory and practice
- Acquire knowledge and learning experimental methods within pharmaceutical/biological and related fields.
- Independently to apply a range of laboratory techniques in biochemistry
Expected learning outcome
The learning objectives of the course are that the student demonstrates the ability to:
- use methods from chemical kinetics and enzyme kinetics to determine characteristic constants such as KM and turnover number for enzyme, both in theory and in practice
- explain how the activity of enzymes are regulated, including allosteric regulation
- explain the structure of biological membranes and how compounds are transported through these
- use the thermodynamic and kinetic foundations of metabolism and explain the significance of free energy and equilibrium constants for coupled reactions and the universal role of ATP in this coupling
- describe the transport of glucose I blood and how it is absorbed
- describe metabolites, enzymes amnd coenzymes in glykolysis, glukoneogenesis, the citric acid cycle and the principles behind regulation and integration of these pathways
- describe the basics of the light reactions of photosynthesis and CO2-fixation in plants (biology students)
Content
The following main topics are contained in the course:
- Chemical kinetics
- Simple Michaelis-Menten kinetics
- Regulation of enzyme activity
- Lipid structures in aqueous solution
- Models of biological membranes
- Active and passive membrane transport
- Ion channels
- Metabolism: basic terms and principles
- Glykolysis and gluconeogenesis
- TCA and oxidative phosphorylation
- Glycogen metabolism
- Integration of metabolism
- Photosynthesis
- Experimental techniques in biochemistry
The students will have the opportunity to study simple biochemical issues through 2 laboratory exercises that lasts 3 hours each:
- exercise: enzyme kinetics
- exercise: metabolism in yeast.
Literature
Laurence A. Moran (Author), Robert A Horton (Author), Gray Scrimgeour (Author), Marc Perry (Author): Principles of Biochemistry, 5th Edition.
See Blackboard for syllabus lists and additional literature references.
Examination regulations
Exam element a)
Timing
Autumn
Prerequisites
| Type | Prerequisite name | Prerequisite course |
|---|---|---|
| Examination part | Prerequisites for participating in the exam a) | N200009101, BMB530: Basic Biochemistry |
Tests
Reports on lab. work
EKA
N200009112
Assessment
Second examiner: None
Grading
Pass/Fail
Identification
Full name and SDU username
Language
Normally, the same as teaching language
Examination aids
To be announced during the course
ECTS value
1
Additional information
The examination form for re-examination may be different from the exam form at the regular exam.
Exam element b)
Timing
January
Tests
Written exam
EKA
N200009102
Assessment
Second examiner: External
Grading
7-point grading scale
Identification
Student Identification Card
Language
Normally, the same as teaching language
Examination aids
No printed or digital aids, only own handwritten notes.
ECTS value
4
Additional information
The written exam is given as a digital exam on Blackboard. A closer description of the exam rules will be posted under 'Course Information' on Blackboard
The examination form for re-examination may be different from the exam form at the regular exam.
Prerequisites for participating in the exam a)
Timing
Autumn
Tests
Participation in lab. work
EKA
N200009122
Assessment
Second examiner: None
Grading
Pass/Fail
Identification
Student Identification Card
Language
Normally, the same as teaching language
Examination aids
To be announced during the course
ECTS value
0
Additional information
The prerequisite examination is a prerequisite for participation in exam element a)
Indicative number of lessons
Teaching Method
The teaching method is based on three phase model.
Intro phase: 22 hours
Skills training phase: 28 hours, hereof: Tutorials: 20 hours and Laboratory exercises: 6 hours
Activities during the study phase:
- Team work for exercises and completion of reports
- Study groups for enhanced learning yield of tutorials
- Deeper understanding of the curriculum during exam preparation
- Preparation for laboratory exercises
Teacher responsible
Additional teachers
| Name | Department | City | |
|---|---|---|---|
| Jonathan R. Brewer | brewer@memphys.sdu.dk | Institut for Biokemi og Molekylær Biologi |