KE825: Nucleic Acids in Medicinal Chemistry and Nanobiotechnology
If there are fewer than 12 students enrolled, the course may. be held with another teaching form.
Students taking the course are expected to: Have knowledge of fundamental chemistry, organic chemistry, biophysics and molecular biology and to be able to use research journals and databases to search for relevant literature.
- Give the competence to designer nucleic acids and their applications in chemistry and biology;
- Impart knowledge of in vitro selection of nucleic acids as artificial enzymes or antibodies;
- Provide an insight into modified oligonucleotides, their structure, properties and related applications in gene therapy and diagnostics;
- Give skills to design new chemical entities or nucleic acid derivatives to address an unresolved specific issue.
Expected learning outcome
- Recognize natural and modified nucleic acids, their secondary structures and possible applications in nanotechnology and gene therapy;
- Understand the principle for chemical and enzymatic synthesis of nucleic acids, and the necessity of chemical modifications for their divergent in vivo applications;
- Perform synthesis, purification and analysis of a model DNA sequence through solid support DNA synthesis, chromatography (column and HPLC) and mass spectrometry techniques;
- Refresh the knowledge of the biological roles of DNA and RNA, in which pathways the nucleic acid-based technology can intervene for potential gene therapy and/or diagnostics;
- Perceive the principle of the in vitro selection of artificial antibodies and enzymes derived from nucleic acids;
- Know the design rationale of two-dimensional and three-dimensional DNA nanostructures, as well as the possible applications.
- Nucleic acid primary structures, synthesis of nucleosides and nucleotides and synthesis of oligonucleotides;
- Nucleic acid secondary and tertiary structures, interactions between nucleic acids and small molecules and proteins;
- RNA-targeting therapeutics (RNA interference, antisense technology and RNA Splicing skipping, etc.);
- DNA-targeting therapeutics (CRISPR CAS9, TFO and strand invasion, etc.);
- The in vitro selection of nucleic acid-based antibodies and enzymes (aptamers and ribozymes, etc.) and the potential applications;
- The design rationale and possible applications of DNA/RNA nanostructures and molecular machines (self-assembly in vitro, DNA origami; imaging);
- Model studies of synthetic nucleic acids in biomolecular targeting in vitro and in vivo (antisense, siRNA, miRNA, aptamer technology);
- Model studies of gene therapy (cellular uptake via lipidation and glycosylation, function of synthetic DNA and RNA oligonucleotides and co-delivery with other modalities).
- Nucleic Acids Book (https://www.atdbio.com/nucleic-acids-book, online resource)
- G. Michael Blackburn and Michael J Gait et al.: Nucleic Acids in Chemistry and Biology, 3rd edition, Royal society of Chemistry. (ISBN: 978-0-85404-654-6)
See Blackboard for syllabus lists and additional literature references.
Exam element a)
Indicative number of lessons
- Intro phase: 16 hours
- Skills training phase: 31 hours,thereof tutorials: 16 hours and laboratory exercises: 15 hours
- preparation for lectures
- individual project
|Chenguang Louemail@example.com||Institut for Fysik, Kemi og Farmaci, Nucleic Acid Center|