BMB836: Application of CRISPR Genome Engineering in Cell Biology and Biomedicine
Students taking the course are expected to:
- Have knowledge corresponding to the curriculum presented in the courses BMB536, BMB507 and BMB508.
- Be able to conduct basic laboratory experiments in cell and molecular biology.
- Give the competence to understand the biology and principles of the CRISPR/Cas9 system
- Give the competence to utilize bioinformatic toolkits to design CRISPR/Cas9 reagents.
- Give experimental skills to apply CRISPR/Cas9 for gene editing
- Give knowledge and understanding of genome engineering in life science research and medical applications.
Expected learning outcome
- Understand the evolution and biological relevance of CRISPR.
- Understand the underlying principles, mechanism of action, and the potential as well as the limitations of genome editing using CRISPR/Cas9.
- Design target specific CRISPR/Cas9 reagents and incorporate their use in own research projects.
- Analyze and assess own results as well as those obtained by others using CRISPR/Cas9.
- Explore other CRISPR-associated enzymes and systems for genome editing.
- Discuss recent developments in the field of genome editing.
- The evolution and biological relevance of CRISPR
- Design of CRISPR/Cas9 reagents (gRNAs) using bioinformatic tools
- Understanding the structure of Cas-proteins and gRNA’s, their associated delivery systems and the principles behind gene targeting
- Delivery methods (transfection/transduction) and associated vector systems
- Basic rules for knockout, knock-in and base editing, including design of knock-in targeting constructs
- The interplay between DNA repair pathways and CRISPR
- Methods for detection of CRISPR/Cas9-induced editing and the establishment and validation of clonal cell lines
- Consideration of on-target and off-target effects and rescue strategies to validate genotypes and phenotypes
- Cell systems and model organisms
- Targeted and genome-wide screens
- Alternative genome editing tools
- Application of the CRISPR/Cas9 system for genome engineering
- Gene therapy and other biomedical applications of the CRISPR/Cas9 technology
- Technology development
- Ethical recommendation
See Blackboard for syllabus lists and additional literature references.
Exam element a)
Oral exam based on an individual written assignment
Indicative number of lessons
At the faculty of science, teaching is organized after the three-phase model ie. intro, training and study phase.
- Intro phase (lectures, classroom teaching) - Number of hours: 16
- training phase: Number of hours: 28 hours, of which 16 hours examination and 12 hours laboratory
Elaboration on the choice of teaching methods and their mutual coherence: The teaching methods for the theoretical part involve lectures based on review articles presented by the instructors and tutorials based on research articles presented by the students and discussed with the instructors. The teaching methods for the practical part involve computer exercise based on open-source software and laboratory exercises. The study phase includes preparations for the theoretical and practical parts and for a final assignment to be defended at an oral exam. The three phases provide progression of knowledge on how to design target specific CRISPR/Cas9 reagents and incorporate their use in the students own research project.
- Prepare for lectures by reading selected literature provided by the instructors.
- Prepare presentations for tutorials, which are conducted in the format of a journal club, where developments in the field of genome editing are discussed by evaluating research publications.
- Prepare small written assignments as part of the computer exercises.
- Prepare a written assignment based on the laboratory exercises (group) and topics discussed during the course (individual).
|Jens S. Andersenemail@example.com||Institut for Biokemi og Molekylær Biologi|
|Kedar Nath Natarajanfirstname.lastname@example.org||Institut for Biokemi og Molekylær Biologi|
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