BMB509: Bioanalytical instrumentation

Study Board of Science

Teaching language: Danish or English depending on the teacher
EKA: N200004112, N200004102
Censorship: Second examiner: None
Grading: Pass/Fail, 7-point grading scale
Offered in: Odense
Offered in: Autumn
Level: Bachelor

STADS ID (UVA): N200004101
ECTS value: 5

Date of Approval: 25-04-2019


Duration: 1 semester

Version: Approved - active

Comment

01012501 (former UVA) is identical with this course description. 

Entry requirements

None

Academic preconditions

Students taking the course are expected to:

  • Have knowledge of basic mathematics and physics that include logarithms and exponential functions, linear algebra, first order differential equations, basic statistics and basic physical principles of electrodynamics, -statics and optics, as taught at gymnasium-level. 

  • Have knowledge of FF502 and FF503, BMB506 Protein chemistry, BMB502 Enzymes and membranes, BMB504 Fundamental Molecular Biology and BMB503 Fundamental Biochemistry as well as 1st-year physics course.

Course introduction

The aim of the course is to enable the student to understand and apply selected methods for investigating biomolecular structure and function using absorption and fluorescence spectroscopy as well as mass spectrometry but also for characterization and functional analysis of biomolecules in living cells using fluorescence microscopy.  The techniques will be demonstrated in practical exercises.

The course builds on the knowledge acquired in the courses listed under academic preconditions below, and gives an academic basis for studying the topics of the bachelor project but also of courses advanced biophotonics and mass spectrometry, that are part of the degree.


In relation to the competence profile of the degree it is the explicit focus of the course to:



  • Give competence in understanding the application of selected biochemical techniques to determine biomolecular structure and function as well as spatial distribution of biomolecules and molecular complexes in cells. 

  • Give skills to apply these techniques to solve a biological problem. 

  • Introduce absorption and fluorescence spectroscopy, fluorescence microscopy and mass spectrometry methods used in modern biochemistry and molecular cell biology, including practical work and demonstrations.

Expected learning outcome

The learning objectives of the course are that the student demonstrates the ability to:

  • Know the principles of frequently applied separation techniques for biomolecules.

  • Sketch experiments for fractionation, isolation and characterization of biomolecules.

  • Interpret straightforward data sets recorded by microscopy, optical spectroscopy or mass spectrometry. 

  • Explain principles of relevant light-matter interaction, as radiation, absorption, reflection, refraction, diffraction or emission from molecules (i.e. fluorescence).  

  • Describe properties and application of fluorescence probes being used in biochemical and cellular research.

  • Know the principles of fluorescence techniques for determining concentrations and properties of biomolecules.

  • Know the anatomy and function of light microscopes including a description of its optical components.

  • Describe the physical principles behind microscopic methods for characterization of cellular structures. 

  • Know how to apply such methods in modern cell biological research.

  • Be familiar with basic principles of digital images and know how to basic image processing and analysis methods.

Content

The following main topics are contained in the course:

  • Introduction to microscope optics and computer-assisted image analysis

  • Separation of biomolecules based on physicochemical properties by chromatography or electrophoresis

  • Introduction into light absorption and fluorescence phenomena as basis for fluorescence spectroscopy and microscopy

  • Detection and characterization of biomolecules by microscopy, spectroscopy, mass spectrometry

  • Visualization and localization of biomolecules in cells by microscopy techniques.

Literature

Notes and articles.
See Blackboard for syllabus lists and additional literature references.

Examination regulations

Prerequisites for participating in the exam a)

Timing

Autumn

Tests

Lab exercises

EKA

N200004112

Censorship

Second examiner: None

Grading

Pass/Fail

Identification

Full name and SDU username

Language

Normally, the same as teaching language

Examination aids

To be announced during the course

ECTS value

0

Additional information

The prerequisite examination is a prerequisite for participation in exam element a)

Exam element a)

Timing

January

Prerequisites

Type Prerequisite name Prerequisite course
Examination part Prerequisites for participating in the exam a) N200004101, BMB509: Bioanalytical instrumentation

Tests

Oral exam

EKA

N200004102

Censorship

Second examiner: None

Grading

7-point grading scale

Identification

Student Identification Card

Language

Normally, the same as teaching language

Duration

20 minutes

Examination aids

Not allowed

ECTS value

5

Additional information

The examination form for re-examination may be different from the exam form at the regular exam.

Indicative number of lessons

46 hours per semester

Teaching Method

The lectures introduce the concepts and set the stage for the lab activities in the training phase.

Activities during the study phase:



  • Read notes, compendia, articles and slides from the lectures

  • Home assignments

  • Laboratory reports

  • Preparation for laboratory exercises

Teacher responsible

Name E-mail Department
Daniel Wüstner wuestner@bmb.sdu.dk

Additional teachers

Name E-mail Department City
Jonathan R. Brewer brewer@memphys.sdu.dk
Thomas J. D. Jørgensen tjdj@bmb.sdu.dk

Timetable

47
Monday
18-11-2019
Tuesday
19-11-2019
Wednesday
20-11-2019
Thursday
21-11-2019
Friday
22-11-2019
08 - 09
Class h2e
Undervisning
09 - 10
Class h2e
Undervisning
10 - 11
Class h1e
Undervisning
11 - 12
Class h1e
Undervisning
12 - 13
Class f
Odense U23
Forelæsning
Class f
Odense U23
Forelæsning
13 - 14
Class f
Odense U23
Forelæsning
Class f
Odense U23
Forelæsning
14 - 15
15 - 16
Show full time table

Administrative Unit

Biokemi og Molekylær Biologi

Team at Registration & Legality

NAT

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