Principles and Applications of PCR Technologies (Summer School)
Course ID
Course Title
ECTS value
Internal Course Code
Responsible study board
Administrative Unit
Date of Approval
Course Responsible
| Name | Department | |
|---|---|---|
| Knud Villy Christensen | kvc@igt.sdu.dk | Institut for Grøn Teknologi |
| Pia Friis Kristensen | piakr@tek.sdu.dk | TEK Fakultetsadministration |
Teachers
Programme Secretary
| Name | Department | City | |
|---|---|---|---|
| Tina Carøe Sørensen | tcs@tek.sdu.dk | TEK Uddannelseskoordinering og -support |
Offered in
Level
Offered in
Duration
Mandatory prerequisites
Two years of studies at university level (equivalent to 120 ECTS) within chemical engineering / biotechnology or similar course programmes, before the summer school starts.
Overall learning objectives
Learning objectives - Knowledge
The student will acquire knowledge on:
- PCR
- Type and design of primers
- Design of thermocycling protocols
- Use of probes
- Analysis of data
- Quantitative PCR (qPCR) and applications
- PCR in synthetic biology and genetic engineering
- PCR to detect contaminants, for phylogenetic applications, culture monitoring and microbiome mapping
- Use of PCR for diagnostics
- Application of PCR in different scenarios in research and industry
Learning objectives - Skills
The student will be able to:
- Design primers
- Test PCR in silico, set up and optimize PCR in the laboratory
- Analyze and interpret results from PCR experiment
- Perform DNA Gel electrophoresis
- Design probes for qPCR
- Perform, design and analyze a qPCR experiment
- Perform RNA extraction purification from mammalian cells or plant cells in the laboratory
- Evaluate purity of the purified RNA
- Synthesize cDNA
- Choice of buffers and the impact of buffers
- Novel advances within PCR techniques and design of probes for qPCR
Learning objectives - Competences
The student will be able to:
- Work sensibly, safely and independently in a PCR lab
- Analyze results from a PCR experiment
- Compile a lab report based on a PCR experiment
Content
URL for Skemaplan
Teaching Method
Number of lessons
Teaching language
Examination regulations
Exam regulations
Name
Examination is held
Tests
Exam
EKA
Name
Description
- Attendance (Minimum 80 % of the time)
- Oral exam : The exam is an oral presentation of a theoretical project on the last days of the course.
Form of examination
Censorship
Grading
Identification
Language
ECTS value
Additional exam information
Additional information
Enrolment is limited to 35 students. If more applicants than places, applicants who meet the mandatory requirements are prioritised according to the below selection criteria:
1. Undergraduate and graduate students from partner universities (exchange); international undergraduates and graduate guest students (fee-paying); undergraduate and graduate students from other Danish universities.
2. Ph. D. Students from partner universities and other international Ph.D. students; other applicants.
Note: Students are prioritised on a first-come-first-served basis i.e. according to the time we receive your complete application. In case a course is filled up, we will try to offer you an alternative course from your list of priorities. All final decisions about admission will be sent out continually.