BMB550. Experimental Life Science

Study Board of Science

Teaching language: Danish
EKA: N200044132, N200044142, N200044152, N200044102
Assessment: Second examiner: None, Second examiner: Internal
Grading: Pass/Fail, 7-point grading scale
Offered in: Odense
Offered in: Autumn
Level: Bachelor

STADS ID (UVA): N200044101
ECTS value: 10

Date of Approval: 03-03-2023


Duration: 2 semesters

Version: Archive

Entry requirements

This course can only be chosen if the course is included as a constituent subject element in your education. I. e. the subject cannot be chosen as an elective subject.

Academic preconditions

Students taking the course are expected to have knowledge corresponding to the requirements for admission to the studyprogram.

Course introduction

The aim of the course is to give the students a broad and inspiring basic introduction to the study of biochemistry, molecular biology and biomedicine. The course will develop and strengthen the students' general study competences and give them theoretical and hands-on introduction to a wide range of basic experimental molecular biology techniques. The course will therefore be competence-developing and form a foundation for subsequent courses in these programmes.

The students will first be introduced to the theory and principles behind the individual methods. This includes an introduction to the physical principles underlying the various experimental techniques through which students are enabled to make calculations and make predictions in, among other things, the following: classical mechanics and electromagnetism. In the experimental part of the course, students will be introduced to various molecular biology techniques based on these physical principles. This will take place in a coherent course, where a biological response in mammal cells is examined. Students will learn the pros and cons of the different techniques as well as gain experience in how results from experiments are analyzed and interpreted. The students will also be introduced to the dissemination of scientific data through both oral and written presentations.

In relation to the competence profile of the degree it is the explicit focus of the course to:
  • knowledge of theory and experimental methods in the biochemical and molecular biological disciplines; 
  • knowledge of the scientific terminology used in the biochemical and molecular biology disciplines; 
  • be able to understand how scientific knowledge is achieved by an interaction between theory and experimentation; 
  • apply one or more biochemical and molecular biology theories and methods; 
  • to be able to carry out analyses using scientific methods and critically reflect on scientific theories and models in the biochemical and molecular biology disciplines; 
  • to acquire new knowledge in an efficient and independent way and to be able to apply this knowledge reflectively; 
  • knowledge of the safety aspects of laboratory work; 
  • describe, formulate and communicate scientific problems and solutions to either peers and non-specialists or collaborators and users; 
  • engage in professional and interdisciplinary collaboration with a professional approach based on group-based project work
  • identify own learning needs and structure their own learning in different learning environments

Expected learning outcome

The learning objective of the course is that the student demonstrates the ability to:

Study technique

  • Use study and learning strategies to plan your own learning process in relation to learning goals, learning activities and evaluation methods
  • Establish professional relations with fellow students and describes ones role as an active contributor to the social and professional environment of the programme.
  • Identifies different science representations (textual, auditory, visual, symbolic, iconic, graphical, tabular, static or dynamic) and applies them in problem solving

Physical concepts

  • Perform simple mathematical derivations.
  • Identify the most relevant components of a physical system.
  • Describe a physical system with a mathematical model.
  • Apply Newton's laws to predict the future behavior of a physical system based on a mathematical model.
  • Calculate electrical and magnetic fields from simple charge distributions and currents.
  • Analyze the movement of charged particles in electrical and magnetic fields.
  • Understand the physical concepts around absorbance and flourescence of light.

Molecular biology techniques

  • .Work independently and safely in a laboratory, including safe handling of chemicals and the most basic apparatus (pipettes, centrifuges, pH meters, etc.).
  • Take notes, work reproducibly, and use standard curves for laboratory work.
  • Understand experimental design and the importance of controls from a theory of science perspective.
  • Perform various basic molecular biology techniques (fx. protein concentration, western blotting and qPCR) and explain the underlying physical and biological principles.
  • Perform and analyze fluorescence microscopy and explain the underlying physical principles.
  • Explain the most basic physical principles behind mass spectrometry and perform simple analyses of proteomics data.
  • Understand the structure of a cell and the flow of information in the cell, including the central dogma.
  • Understand how different experimental methods can be used to analyze a cell's response to extracellular stimuli.
  • Relate theoretical knowledge to practical experiments and observations.
  • Clarify and analyze a scientific problem in a ready-made form and communicate about both the solution process and its results.

SDGs

  • Understand the SDGs and their relationship with academic education
  • Understand issues and dilemmas related to achieving the SDGs
  • Work academically, critically reflected and interdisciplinary with the SDGs
  • Reflect on the relevance of the SDGs to biochemistry, molecular biology and biomedicine, as well as in relation to other disciplines


Content

The following main topics are contained in the course:

Study technique

  • Study group: Work in collaborative groups, communication, planning, conflict management, group forming and constitution and study group contract
  • The student’s learning: Introduction to e-learning, laboratory security, study and learning strategies, harmonization of expectations, ethical standards in academia and the student’s personal learning plan.
  • Problem solving anchored in topics from the student’s introductory courses.

Physical concepts

  • Physical quantities and units.
  • Movement and kinematics.
  • Newton’s laws, forces and momentum.
  • Movement and rotation of rigid bodies.
  • Basic electromagnetism.
  • Properties of light including refraction and absorption.

Molecular biology techniques

  • Buffer calculation and manufacturing.
  • Standard curves.
  • Organelle purification using centrifugations.
  • Introduction to selected molecular biology techniques and analyses, e.g.
  •        1.Western blotting.
  •        2. Fluorescence microscopy.
  •        3.RNA purification, cDNA synthesis and qPCR.
  •        4.Analysis of mass spectrometry/proteomics data.
  •        5.Analysis of quantitative data.

SDGs (self-study, online)

  • A research-based introduction to sustainability world goals and their relevance to academic studies
  • Introduction to the SDGs and their historical and political context
  • Specific problems associated with the SDGs
  • Dilemmas as the work to achieve the SDGs arises
  • Providing a cross disciplinary platform to work with the SDGs
  • Experience with working critically and reflected with the SDGs.

Literature

See itslearning for syllabus lists and additional literature references.

Examination regulations

Exam element a)

Timing

Autumn

Tests

Compulsory assignments in the study introduction programme

EKA

N200044132

Assessment

Second examiner: None

Grading

Pass/Fail

Identification

Full name and SDU username

Language

Normally, the same as teaching language

Examination aids

Allowed, a closer description of the exam rules will be posted i itslearning.

ECTS value

1

Additional information

The assignment consists of:

  1. Submitted study group contract which must be approved.
  2. Compulsory participation in laboratory safety course
  3. Spot test in laboratory safety, which must be completed with at least 80% correct answers. The test can be taken several times before the deadline. Every time the test is submitted, feedback is given to the student.
  4. An e-test which must be completed with at least 80% correct answers. The test can be taken several times before the deadline. Every time the test is submitted, feedback is given to the student.

Exam element b)

Timing

Spring

Tests

Compulsory task in sustainable development SDGs

EKA

N200044142

Assessment

Second examiner: None

Grading

Pass/Fail

Identification

Full name and SDU username

Language

Normally, the same as teaching language

Examination aids

Allowed, a closer description of the exam rules will be posted i itslearning.

ECTS value

1

Additional information

The assignment consists of the completion of an E-learning process during Spring. As part of the plans in itslearning, it must be stated when the various parts of the e-learning process are to be completed. In order to pass, all modules must be completed.

Exam element c)

Timing

Autumn and January

Tests

Portfolio

EKA

N200044152

Assessment

Second examiner: None

Grading

Pass/Fail

Identification

Full name and SDU username

Language

Normally, the same as teaching language

Duration

MCQ exam - 2 hours

Examination aids

The MCQ exam is without aids. However, it is allowed to use the collection of formulas that is handed out electronically. In addition, use of the standard build in calculator in Windows/MAC is allowed. Furthermore, it is allowed to use Maple, MathCad, Matematica, GeoGebra Apps, R, R-Studio, CAS TI-Nspire, Microsoft Excel or Libre Office Calc. The us of WordMat is allowed, but not recommended. The use of WordMat is at one's own risk, and there will be no support if use of the program causes errors or break downs. 

Internet is not allowed during the exam. However, you may the course site in itslearning in connection with filling out the multiple-choice questions.  

ECTS value

4

Additional information

Portfolio exam consisting of three elements: 

1. Laboratory exercises
2. Oral presentation
3. MCQ exam without aids in January.
All three elements have to be passed in order to obtain a final pass grade

Laboratory exercises are passed by participation in the exercises. If one or two exercises have been missed, an oral exam will be held in week 51 by the teacher, which will cover the content of the missing exercises.
Oral prensentation is passed by participating in the oral group presentation of the lab results during SFV classes. I the oral group presentation is missed, then there will be an individual oral presentation with the teacher in week 51.
The MCQ exam is passed by obtaining a passing grade in the MCQ exam in January
The reexam will be a written exam without aids consisting of both MCQ and essay questions, but will be oral with 20 or less participants. The oral exam is without aids and preparation. Duration is 20 minutes incl. voting. The student will be assigned a topic and will the present the assigned topic. The presentation will be used as a departure point for questions, but questions can be posed in entire syllabus.

Exam element d)

Timing

Spring and June

Tests

Port folio

EKA

N200044102

Assessment

Second examiner: Internal

Grading

7-point grading scale

Identification

Full name and SDU username

Language

Normally, the same as teaching language

Duration

MCQ exam - 2 hours

Examination aids

The exam in June is without aids. However, it is allowed to use the collection of formulas that is handed out electronically. In addition, use of the standard build in calculator in Windows/MAC is allowed. Furthermore, it is allowed to use Maple, MathCad, Matematica, GeoGebra Apps, R, R-Studio, CAS TI-Nspire, Microsoft Excel or Libre Office Calc. The us of WordMat is allowed, but not recommended. The use of WordMat is at one's own risk, and there will be no support if use of the program causes errors or break downs. 

Internet is not allowed during the exam. However, you may the course site in itslearning in connection with filling out the multiple-choice questions.  

ECTS value

4

Additional information

Portfolio exam consisting of these three elements:

  1. Lab exercises
  2. Written group assignments
  3. MCQ exam without aids in June

All elements must be present in order to pass the exam.

Lab exercises are passed by attending lab exercises. If one has not attended one or two lab exercises, then one must attend an oral exam with the teacher covering the content of the missed lab exercises.

Distribution of exams and points are as follows: Written groups assignments 30% total, MCQ exam in June: 70%. Internal marking by teacher. When all exams have been completed a final grade is given, based on the combined number of points obtained. Grades given according til the 7-mark scale. (01013502). Participation in both Lab exercises, written group assignments and the MCQ exam in June is obligatory to obtain the grade 02 or higher.

The reexamination will be a written exam without aids consisting of both MCQ and essay questions. The reexam will change to an oral exam if 20 or less students have enrolled. The oral exam is without aids or preparation. 20 minutes including grading. The student will pull a topic, and will immediately afterwards commence with presenting this topic. Questions for the student will initially be based on the selected topic, but can be in the entire syllabus for the course.

Indicative number of lessons

125 hours per semester

Teaching Method

At the faculty of science, teaching is organized after the three-phase model ie. intro, training and study phase.
  • Intro phase: 46 hours
  • Training phase: 79 hours, hereof tutorials: 35 hours and laboratory exercises: 34 hours

The intro phase is two-part consisting of physics lectures, where an introduction is given to various physical subjects, after which a biological lecture explains different laboratory techniques based on the introduction of the physical subjects. In addition, subsequent laboratory or tutorial exercises are also introduced in selected techniques.

The training phase consists of physics tutorials and biology laboratory exercises and tutorials in smaller teams. Priority is given to "active learning" in tutorials, where students are involved and encouraged to participate actively in the teaching to the highest possible extent, e.g. when solving tasks in groups or other work in groups or independent work. For laboratory exercises, students work in pairs or smaller groups with practical exercises within the subject areas of the course.

During the study phase, the aim is to ensure that the students work with the subjects in a different way than regular tutorials. The study phase may be used for preparation for tutorials or work may be directly reported from the study phase in tutorials. Cooperative learning is thought of as part of the study phase.

Activities in the study phase:

  • Work in study groups (planned study phase), including the use of cooperative learning
  • Preparation for tutorials
  • Preparation of presentation and laboratory report

Teacher responsible

Name E-mail Department
Rasmus Siersbæk siersbaek@bmb.sdu.dk Institut for Biokemi og Molekylær Biologi
Søren Fisker Schmidt sfs@bmb.sdu.dk Funktionelle Genomiske Studier og Metabolisme

Additional teachers

Name E-mail Department City
Søren Sten Hansen shan@sdu.dk Det Naturvidenskabelige Fakultetssekretariat

Timetable

Administrative Unit

Biokemi og Molekylær Biologi

Team at Educational Law & Registration

NAT

Offered in

Odense

Recommended course of study

Transition rules

Transitional arrangements describe how a course replaces another course when changes are made to the course of study. 
If a transitional arrangement has been made for a course, it will be stated in the list. 
See transitional arrangements for all courses at the Faculty of Science.