BMB207: Biophotonics
Study Board of Science
Teaching language: English
EKA: N220009112, N220009102
Assessment: Second examiner: None
Grading: Pass/Fail
Offered in: Odense
Offered in: Spring
Level: PhD
STADS ID (UVA): N220009101
ECTS value: 5
Date of Approval: 23-10-2018
Duration: 1 semester
Version: Archive
Comment
The course has limited entry. The following criterias are taken into consideration when seats are assigned.
- PhD students after time of enrollment
- Students with the most ECTS from their master
- Students who follows master courses concurrent with their bachelor programme (dispensation to 30 ECTS Master courses)
If students are equal – seats are allocated based on random draw.
The academic envirometns at The faculty of science manages the prioritisation and at waiting list is established and will then be made aware from the faculty. The waiting list will not be transferred to the following year.
Entry requirements
Master degree in biology, chemistry, physics, bio chemistry and molecular biology, biomedicine, medicine or nanobioscience.
It is not possible to take the course if you have already taken BMB825.
It is not possible to take the course if you have already taken BMB825.
Academic preconditions
Participant limit
Course introduction
It is the goal of the course to teach the students general principles of
fluorescence and light microscopy as well as teach principles behind
advanced fluorescence and imaging techniques as well as a short
introduction to image processing. There will be practical demonstrations
and hands on exercises on all topics. The course is a five day
intensive course taking place within one week.
fluorescence and light microscopy as well as teach principles behind
advanced fluorescence and imaging techniques as well as a short
introduction to image processing. There will be practical demonstrations
and hands on exercises on all topics. The course is a five day
intensive course taking place within one week.
In relation to the competence profile of the degree it is the explicit focus of the course to:
- Give the competence to Identify the “method of choice” for analysis of a given biological sample
- Give skills to perform image analysis using advanced microscopy instrumentation
- Give knowledge and understanding of the strengths and limitations of modern techniques in microscopy
Expected learning outcome
The learning objective of the course is that the student demonstrates the ability to:
- Understand the basics of microscopy and optical bioimaging.
- Discuss strengths and limitations of modern techniques in microscopy.
- Identify the “method of choice” for analysis of a given biological sample
- Perform image analysis using advanced microscopy instrumentation
- Present scientific results in an oral seminar given in English
- Evaluate scientific results in the original research literature
Content
The following main topics are contained in the course:
- Wide field microscopy
- Laser scanning single-photon confocal microscopy
- Two-photon microscopy
- Spinning disk confocal microscopy
- Fluorescence Correlation Spectroscopy (FCS)
- Raster Image Correlation Spectroscopy (RICS)
- Fluorescent Lifetime Imaging microscopy (FLIM)
- kICS/PALM (k‐Space Image Correlation Spectroscopy/ photoactivated localization microscopy)
- Coherent anti Raman scattering microscopy (CARS)
- Stimulation emission depletion microscopy (STED)
- Introduction to image analyses using FIJI and Huygens
Literature
Examination regulations
Prerequisites for participating in the exam element a)
Timing
Spring
Tests
Participation
EKA
N220009112
Assessment
Second examiner: None
Grading
Pass/Fail
Identification
Student Identification Card
Language
Normally, the same as teaching language
Examination aids
To be announced during the course
ECTS value
0
Additional information
Participation in all lectures, laboratory exercises and tutorials.
The prerequisite examination is a prerequisite for participation in exam element a)
Exam element a)
Timing
June
Prerequisites
| Type | Prerequisite name | Prerequisite course |
|---|---|---|
| Examination part | Prerequisites for participating in the exam element a) | N220009101, BMB207: Biophotonics |
Tests
Oral exam
EKA
N220009102
Assessment
Second examiner: None
Grading
Pass/Fail
Identification
Student Identification Card
Language
Normally, the same as teaching language
Examination aids
To be announced during the course
ECTS value
5
Indicative number of lessons
Teaching Method
At the faculty of science, teaching is organized after the three-phase model ie. intro, training and study phase.
- Intro phase (lectures, class lessons) - 12 hours
- Training phase: 29 hours, including 8 hours tutorials and 21 hours laboratory
Educational activities
- Literature studies
- Preparation of project individually and in groups.
Teacher responsible
| Name | Department | |
|---|---|---|
| Jonathan R. Brewer | brewer@memphys.sdu.dk | Institut for Biokemi og Molekylær Biologi |